Abstract: An efficient regeneration protocol has been developed for rapid and mass scale clonal propagation of Lavendula officinalis from shoot tip explants. For the induction of multiple shoot formation, the explants were cultured on Murashige and Skoog`s (MS) basal medium supplemented with different concentrations of cytokinins viz., BAP (0.5-2.0 mg L-1), Kn (0.5-2.0 mg L-1) and on different cytokinin plus auxin combinations viz., BAP (1.0-2.0 mg L-1+IAA (0.5-1.0 mg L-1) and Kn (1.0-2.0 mg L-1) + IAA(0.5-1.0 mg L-1). These shoot tips produced multiple shoots, with the best result in BAP (2.0mg L-1) whereby (45±6.40) shoots were produced/explant in 80% cultures in 4 weeks time. The regenerated shoots were subcultured on the best induction medium (BAP 2.0 mg L-1) after every 4 weeks so as to increase their number. These shoots rooted best in half strength MS basal medium fortified with IBA (1.0mg L-1) with 7-18 roots per shoot(Avg. 16±6.89) roots in 80% cultures in 4 weeks time. Rooted plantlets were shifted to the plastic pots containing mixture of sand:clay:vermiculite (1:1:1) and kept for two weeks under controlled conditions of temperature (25±2°C) and relative humidity 60%. Afterwards these plantlets were hardened in green house environment (Vista Biocell Limited ) for one month and finally shifted to field conditions were 70% plants survived.