Abstract: Identification of the parent combinations with long genetic distance and desirable HMW-GS compositions is one of the most important steps for improving bread-making quality in bread wheat. We used RAPD and seed storage protein markers for identifying the best parent combinations of 28 Iranian wheat cultivars and advanced breeding lines for improving bread-making quality in wheat. We considered genetic distance less than 0.1 (<0.1) and quality score equal to or more than eight as criteria for selecting the best parent combinations. Six combinations out of studied cultivars including Khazar1/Darab1, Azadi/Darab1, Chenab/Darab1, Navid/Darab1, Star/Darab1 and Deihim/Darab1, were considered as suitable parents for future crosses in wheat breeding programs for breed-making quality in Iran. We suggest it should also be considered other important factors such as parents performance and complementarity for important agronomic traits in parent selection. However, integrated data of RAPD and HMW-GS compositions can make useful parent selection for breeding programs related to bread-making quality.