Abstract: Arbuscular Mycorrhizal (AM) fungi are of considerable interest because of their ability to form symbiotic associations with 85% plants and their potential to use as a biofertilizer. Rhizosphere soil and roots samples of wheat and maize were collected from marginal and fertile soils of Hazara division from soil series extensively use for the production of wheat and maize crops. Physicochemical characteristics of the soil under investigations were determined. AM fungal spores were isolated from soil, identified and their infections rates in the roots were determined. Data indicated that soil pH values of Hazara division ranged from 6.38 to 7.66, soil organic matter content ranged from 1.20 to 1.97% and lime from 1.5 to 11.5%. Maximum numbers of AM fungal white spores were found in Battagram and Abbottabad, brown spores in Chamba and black spores in Abbottabad and Chamba soil series in fertile soil whereas in marginal soil, high numbers of white spores were found in Dedal, Battagram and Abbottabad, brown spores in Dedal and Mansehra and black spores in Chamba, Jaba and Haripur soil series of this area. In fertile soil, 8 to 43% AM fungal infection rates were noted in roots of wheat crop where as 44 to 56% infection rates were observed in marginal soil of this area. Results suggest that comparatively higher AM fungal spores and their root colonization in wheat and maize crops were observed in marginal soil than fertile soil with varied spores density and infections intensity from one site to another. More AM fungal spores density caused higher roots infection intensity in wheat and maize crops of the area and higher AM infections rates were observed in soil of around neutral pH values with low organic matter contents.