Abstract: Rauvolfia tetraphylla L. is an economically important medicinal plant because of the presence of various alkaloids, which are in use of cardiovascular and psychiatric treatments. Flowering is a complex process up regulated by external and internal factors and its induction under aseptic in vitro culture is extensively rare. Nodal segments and shoot tips were cultured on MS medium supplemented with various concentrations of BAP and GA3. Maximum number of multiple shoots (4.56±0.098) was obtained at 4.44 μM of BAP after two weeks of inoculation. When GA3 was used in combination with BAP, in vitro flowering was observed. Flower bud production and flower induction was noticed at 4.44 μM of BAP with 4.3 μM of GA3. However, maximum number of shoots was reduced to three in the same concentration.