Abstract: Two different sequences of Echinococcus granulosus Antigen B, (A major hydatid cyst fluid antigen), acquired from Gene Bank and amplified via RT-PCR reaction. The amplified fragments (HI, HII) cloned into pTZ57R vector by T/A cloning and subcloned into pGEMEX-1 expression vector. The subcloned genes were expressed by IPTG. To confirm the expression of subcloned genes, The SDS-PAGE performed and production of about 35 KDa recombinant fusion proteins were confirmed for either two cloned genes. The immunoreactivity of the recombinant fusion proteins were tested using double diffusion and immunoblotting. Both recombinant fusion proteins derived from lysate of transformed bacteria, were reactive for antibodies in serum of cystic hydatid patient.