Abstract: This study was conducted to reveal the levels of endogenous GA3 in different parts of artichoke (Cynara scolymus L.). Cynara scolymus L. cv. Sakiz, an important Turkish cultivar, was used as plant material. The levels of endogenous GA3 on GA3 treated and non-treated plants (control) were determined by High Performance Liquid Chromatography (HPLC) method. Experiment results revealed that the highest level of GA3 was present on the plants treated with in 25 ppm GA3 and had immature head. On the other hand it was found that the lowest level of GA3 was present on the plants treated with 50 ppm GA3 and had mature head.