Abstract: In this study the effect of three genotypes (KalG, Kal-early and Su2270 as the commercial cultivars in Iran), two explants (cotyledon and hypocotyl), growth regulators (BAP, NAA and Zeatin) and a strain of Agrobacterium tumefaciense (pGV3850) on regeneration and transformation efficiency is reported. Although all cultivars and explants were regenerated on the different media, however our results showed that there were significant differences between genotypes and explants in the number of shoot formation per explant. Optimal regeneration of KalG, Kal-early and Su2270 cultivars were observed on MS media supplemented with 2 mg L-1 Zeatin and 2 mg L-1 BAP, respectively. The maximum number of shoot formation per explant was observed in cotyledon and hypocotyle explants in KalG and Su2270 cultivars, respectively. The transformation rate was 17% for Kal-early to 35% for KalG cultivars. Transformation were confirmed by Gus assay and PCR analysis.