Abstract: Improving an identification method was purposed using electrophoretic profiles of seed proteins and four isoenzymes of Karaelci and Sarielci varieties of Vicia sativa L. In addition five different extraction buffers were tested for seed proteins. Also seed proteins and four isoenzymes polymorphism between two varieties were investigated. Electrophoresis was carried out with polyacrylamide gel electrophoresis (PAGE). Catechol oxidase and α-glycerophosphate dehydrogenase isoenzymes exhibited identical patterns but esterase and superoxide dismutase isoenzymes showed different patterns. These two isoenzymes, which showed polymorphism, can use as marker suchlike studies with these varieties. Seed proteins band patterns among five buffers were different. One of the buffers revealed maximum band numbers. This buffer was the best seperator buffer. Isoenzymes and seed proteins band patterns which find out can be useful for identification.