Abstract: Avian Influenza (AI) has been recognized as a highly contagious and lethal generalized viral disease of birds. In this study, immune response of layers to the commercial oil based and liposomal vaccines of avian influenza H7 was evaluated. Thirty commercial layers were divided into three groups, T1, T2 and T3 with 10 birds in each group. Group T1 served as control, Group T2 was immunized with conventional AI oil-based vaccines, 0.5 mL/bird and Group T3 was immunized with AI Liposomal vaccines 0.5 mL/bird through sub/cut injection. Blood samples were taken and sera were separated at day 0, 7, 14, 21, 28 and day 35. Each time at least 6 samples were taken for antibody titration through AGPT. The geometric mean titre (GMT) of birds in T1, T2 and T3 was 4 ±1.02 at day 0. No significant difference was observed in the titres at day 0 in all the groups. The GMT (Geometric Mean Titer) of control group was 4 ±1.02 at day 7, 6.79 ±1.02 at day 14 and 8 ±1.02 from day 21 to 35. The antibody titre increased slowly from 32 on day 7 to 630.3 on day 35 in birds vaccinated with oil based vaccine, whereas a somewhat quick increase in immune response from 64 on day 7 to 891.4 on day 35 was observed in birds vaccinated with liposomal vaccine. The results showed that immune response of layers in term of GMT was well established with AI liposomal vaccine as compared to that of oil based vaccine. The present study will be helpful in preventing the commercial losses of the farmers and preventing the flock mortality due to the high efficacy of liposomal vaccine against avian influenza.