Abstract: This study was performed to determine the biochemical changes in cerebrospinal fluid (CSF) of the dogs exposed to trauma. Cerebrospinal fluids were collected from 12 nontraumatic and clinically healthy dogs and 12 traumatic dogs aged between 1 and 36 months for determination of AST, ALT and CK activities, Ca, Pi, Mg, glucose and creatinine levels. Compare to healthy dogs, no differences were determined in AST activity, Ca and Mg levels in traumatic dogs. However CK and ALT activities, glucose, Pi and creatinine levels increased. In conclusion, the biochemical changes in CSF of traumatic dogs may be of practical value for monitoring the prognosis and therapy.