Abstract: In this study, the collecting date of softwood cuttings, rooting media, ortet age and cutting type were investigated on Alnus glutinosa subsp. barbata (C. A. Mey.) Yalt. Rooting media and cutting type showed no significant effect on the rooting percentage (RP), ramet height (RH) and rootlet number (RN) while ortet age showed significant effect on these parameters. The highest RP as 56.7 and 48.3% were obtained for the tip cuttings and basal cuttings of 1+0 aged ortets, respectively, while the lowest RP as 0%, was obtained for the tip cuttings of 2+0 aged ortets. Determining of the lower RP for the cuttings of 2+0 aged ortets than the cuttings of the older ortets can be explained together with the ortet genetic character. The highest RH (18.3 cm) was obtained for the tip cuttings of the 1+0 aged ortets and RN (8.3 number) was obtained for the basal cuttings of the same ortets. The highest mean RP were obtained for the cuttings of 1+0 aged seed originating ortets and physiologically 1+0 aged sprout originating ortets as 41.35 and 35.55%, respectively. Based on the results of this study, basal cuttings of the young ortets, collected in the middle of the June should be used in the autovegetative propagation of Alnus barbata.