Abstract: Spontaneous hybridization in wild wheat species are frequently observed in natural flora of Turkey. The aim of this study is to investigate the incidence of hybridization in Aegilops L. species under natural conditions. Aegilops populations of 3 different ploidy level, collected from south east Turkey were sown in mixed plots with tetraploid and hexaploid wheat land races of the same origin. Harvested seed materials were sown in the following year to see incidence of hybridization. Out of 73 Aegilops entries, 16 of them consisting of 5 polyploid species gave hybrid individuals. Hybrids were characterized for 5 traits to check similarity between them. Significant differences and similarities were observed among the hybrids, in terms of the measured and observed characters. Results of the study suggest that introgression from the relatives into Aegilops species is quite possible. Another point to underline is many of the seed multiplication programs do not mind space isolation between self fertilizing species of wild and cultivated wheat species. Accomplishment of seed multiplication and rejuvenation studies may require some degree of space isolation to avoid contamination between populations.