Abstract: The present study were to evaluate the effect of individual bull, semen types, quality of bull semen, sources of semen on Non-return Rate (NRR) at 60 days of cows under field condition. A total 75550 cows were inseminated with 71 bull semens from Central Cattle Breeding Station and Dairy Farm (CCBSDF), Savar, Dhaka, Rajshahi Dairy and Cattle Improvement Farm (RDCIF), Rajabarihat and District Artificial Insemination Centre (DAIC), Rajshahi under 40 Artificial Insemination (AI) sub-centres/points of District AI centre, Rajshahi. The overall NRR was obtained 78.54% with chilled and frozen semen produced from three AI centres/stations. Analysis of variance showed that individual bull semen had significant (p<0.05) effect on NRR at 60 days after first insemination. Semen types, quality of bull semen and sources of semen had significant (p<0.001) effect on NRR at 60 days of cows. The significant (p<0.001) highest NRR (82.32%) was with chilled semen and lowest was with frozen semen (76.39%). The significant (p<0.001) maximum NRR (83.12%) was for the best quality bull semen and minimum (70.13%) for the poor quality bull semen. Significant (p<0.001) higher NRR (82.32%) was in semen from DAIC, Rajshahi and lower (73.01%) in semen from RDCIF, Rajabarihat. Results suggested that the NRR of cows at 60 days after first insemination under field condition may be a good practice to discard poor fertility semen among the individual bull semen, semen types (chilled and frozen), quality of bull semen (poor, good and best) and sources of semen (CCBSDF, Savar, RDCIF, Rajabarihat and DAIC, Rajshahi) for artificial insemination programme in Bangladesh.