Abstract: Germination of Artemisia herba alba seeds in the presence of esculetin showed lower G6PDH or 6PGDH activities compared to that germinated in esculetin absence; however, the same enzymes showed higher activities upon germination in the dark compared to that under light. Both enzymes were continuously increased from day one to day five during germination. Of the isoenzyme pattern found G6PDH or 6PGDH, the fast moving band of G6PDH or the slower moving band of 6PGDH was influenced by germination under dark condition or presence of esculetin.