Abstract: The study was conducted at the Agricultural Biotechnology Research Labs, National Agricultural Research Centre, Islamabad to ascertain varietal differences between four wheat varieties (Arz, Pak-81, LU-26 and Pavon); with respect to their tolerance to sodium chloride (NaCl) stress indicated by callogenesis and organogenesis. Murashige and Skoog (MS) modified medium supplemented with 50, 100, 150 and 200 (mM) sodium chloride salt was used for tissue culture. Results clearly indicate striking differences among different wheat varieties in callus induction and regeneration under salinity stress. LU-26 was the most tolerant cultivar followed by Pavon, Pak-81 and Arz. The findings can help in breeding for salt tolerance in wheat.