Abstract: The objective of this study is to investigate the production of Acetone, Butanol and Ethanol (ABE) by immobilized cells of C. saccharoperbutylacetonicum N1-4 (ATCC 13564) under anaerobic batch culture system. Two different methods of immobilization, active immobilization in alginate and passive immobilization by employing stainless steel scrubber, nylon scrubber, polyurethane with uniform pore’s size, polyurethane with different pore’s size and palm oil empty fruit bunch fiber were studied. Immobilization in alginate was carried out on the effect of cell’s age, initial culture pH and temperature on the production of ABE. Immobilized solventogenic cells (18 h) produced the highest total solvents concentration compared to the other phases with productivity of 0.325 g L-1 h-1. The highest solvents production by active immobilization of cells was obtained at pH 6.0 with 30°C with productivity of 0.336 g L-1 h-1. Polyurethane with different pore’s size is significantly better than other materials tested with production of solvents productivity and YP/S at 3.2 times and 1.9 times, respectively compared to free cells after 24 h fermentation. We conclude that passive immobilization technique increases the productivity (215.12 %) and YP/S (88.37 %) of solvents by C. saccharoperbutylacetonicum N1-4.