Abstract: To date 244 species of spiders belonged to 33 families have been recorded from Iran. Sixty two species, 4 genera and 8 families are reported for the first time from Iran in 2002-2006. Spiders were collected from branches, leaves, flowers and trunks of trees and bushes, on the ground, under the stones and grasses by Steiner and Baggiolini method, bottle, aspirator, pitfall trap, pans and insect net. Geolycosa altera Roewer, 955 was found to be junior synonym of Honga radiata (Latreille, 817). Four species of Lycosid spiders were transferred to Trochosa: T. austrocaspia (Roewer, 955), T. flavichelis (Roewer, 955), T. nava (Roewer, 955), T. persia (Roewer, 955).