Abstract: Seed samples of 20 indigenous and developed mustard/rapeseed cultivars (Brassica campestris, B. juncea and B. napus) were analyzed for tocopherol- and sterol-content. Gas liquid chromatography and high performance liquid chromatography were used, respectively, for sterol and tocopherol analysis. Four types of tocopherols were found in mustard oil in various amount: α-tocopherol, β-tocopherol, χ-tocopherol and δ-tocopherol, ranged from 14.96 to 32.48, trace amount to 1.19, 26.12 to 66.51 and 2.02 to 5.38 μg g-1 lipids, respectively. Only χ-tocotrienol, among the tocotrienlos, was identified and its amount varied from 2.02 to 7.49 μg g-1 lipids among the cultivars. Major sterols identified and their lipid concentrations in μg g-1 lipid were as follows: sitosterol, 3,183 to 4,463; brassicasterol, 582 to 1,368; campesterol, 1,306 to 3,471; Δ5-avenasterol, 138 to 1,135 and cycloartenol, 119 to 1,170. This study shows that both tocopherol and sterol content differ significantly among the cultivars.