Abstract: In this research seedling samples, taken from seed germination, have been studied. Polyacrylamide gel electrophoresis of some enzyme systems have been done. The resulted bands were analyzed by computer. These analyses have been performed by NTSYS-pc software, using UPGMA (Unweighted Paired Group Method Arithmetic average) method and on the basis of Jaccard correlation coefficient. Results show that in the first step the species H. pusillus and in the second step H. insanus are separated from the other species. The species H. arachnoideus and H. niger are very heterogenous. We observe that species relationships depend on the locality of plant samples. Therefore, the study of interspecific relationships depends on the study of intraspecific variation.