Abstract: Wheat (Triticum aestivum L.) and jointed goatgrass (Aegilops cylindrica Host) are genetically related. Both species share the D-genome, allowing hybrids between them to easily form under natural and controlled environments. These hybrids are 100% male sterile but partial female fertile. The objective of this study was to produce hybrids and amphiploids of Ae. cylindrica and T. aestivum and test them for male and female fertility. Hybrids between wheat and jointed goatgrass were produced in the greenhouse. Of the 24 plants only sixteen germinated. All germinated wheat H jointed goatgrass hybrids were treated with colchicine to produce amphiploids. Root-tip chromosome counts in the treated plants showed 70 chromosomes in 6 (37.5%) plants and chimeric numbers (35 and 70 chromosomes) in 3 (18.7%) plants while the remaining 7 plants had 35 chromosomes. Amphiploids of Ae. cylindrica H T. aestivum, produced by colchicine treatment, were 100% male and female sterile and did not produce any seeds. Moreover, phenotypically amphiploids of wheat H jointed goatgrass looked similar to normal wheatHjointed goatgrass hybrids with 35 chromosomes. Possible reasons for the sterility in amphiploids could be due to cytological instability and/or genome dosage.