Abstract: Nodal explants of Mentha arvensis L. from naturally grown plants were cultured on MS basal medium fortified with various combinations of auxins and cytokinins. The best response for multiple shoot regeneration (4±1.58) and callogenesis was observed in MS+4.4 μM BAP. Whereas, explants cultured on MS+8.9 μM BAP+4.4 μM IAA exhibited the best direct multiple shoot regeneration (14±2.74) and rhizogenesis (1±0.70). The well developed micro shoots were successfully acclimated.