Abstract: In order to investigate the relationship between hybridization compatibility and genetic distances and to provide genetics evidence for obtaining bridge species for the introgression from S. lycopersicoides to L. esculentum, 48 materials including 5 genotypes from S. lycopersicoides and 43 genotypes from Lycopersicon (9 species) were used in the present study. Genetic distances among S. lycopersicoides and 9 species from Lycopersicon were estimated by morphological markers and Random Amplified Polymorphic DNA (RAPD) markers, respectively and the results showed that genetic distances ranged from 0.304 to 0.406, with the Morder being L. chilense, L. peruvianum, L. glandulosum, L. pimpinellifolium, L. chmielewskii, L. hirsutum, L. cheesmanii, L. esculentum, L. pennellii and L. parviflorum from near to distant. Furthermore, hybridization compatibility of L. chilense, L. esculentum and L. pennellii with S. lycopersicoides (as male parent) was also studied and the results showed that pollen germination and pollen tube elongation were different among hybrid combinations.