Abstract: A micropropagation method was developed for Anthemis xylopoda O. Schwarz (Asteraceae), a critically endangered endemic species of Turkey to provide an efficient alternative propagation method. Seeds of Anthemis xylopoda which were collected from natural habitat were germinated on Murashige and Skoog medium (MS) supplemented with different concentrations of Gibberellic Acid (GA3). The highest percentage germination was observed on MS medium with 1 mg L -1 GA3. Shoots obtained from in vitro germinated seeds used as initial explant for shoot multiplication experiments. Shoot multiplication was achieved on MS medium with different cytokinins. The highest number of shoots per explant was obtained on MS medium containing 0.005 mg L -1 Thidiazuron (7.83 per explant). The highest mean of maximum shoot length was found MS without growth regulators and Kinetin (0.1 mg L -1) at low concentration. Elongated shoots (~3 cm), excised from multiple shoot cultures were transferred to rooting media. Indole-3-butyric acid (IBA) was more effective than Indole-3-acetic acid (IAA) for the root induction. The best rooting result (60%) was obtained on the MS medium with 0.5 mg L -1 IBA. The plantlets with well developed root were transferred to ex vitro conditions. About 70% of the rooted shoots were successfully established in pots after hardening.