Abstract: Random Amplified Polymorphic DNA (RAPD) analyses was undertaken to assess genetic relationships among isolates of Corynespora cassiicola, a destructive fungal pathogen of many economically important crop plants including tomato, cucumber, perilla, rubber, etc. from different host plants. Of the 8 random primers tested, two were selected that generated reproducible amplification pattern using three isolates representing each pathogen. RAPD-PCR also amplified reproducible polymorphic bands. A total of 64 bands were scored, 12 of which were polymorphic. RAPD analyses revealed that the amplification products were similar among isolates of each pathotype from the different host plants.