Abstract: By use of selective media, putative antagonist bacterial isolates were isolated from rhizosphere-associated soils from different parts of the Amik plain. Isolates to be tested were chosen in approximate proportion to their abundance on the plates. Selected 113 bacterial isolates were screened for in vitro antagonism towards two important soil-borne root infecting plant pathogens, Sclerotinia sclerotiorum and Rhizoctonia solani by using dual culture test. From this test, significant numbers of bacterial isolates, but not the yeast, were found to produce inhibition zones by inhibiting the hyphal growth of fungal pathogens to a varying degree. On the basis of the result obtained from the preliminary screening test, isolates belonging to Bacillus spp. and Pseudomonas spp. found to be highly efficient against fungal pathogens used in the experiment. Among the inhibitory isolates, bacterial isolates AKB50 and AFP104 significantly suppressed hyphal growth of S. sclerotiorum and R. solani by 75.3 and 83.3%, respectively. This study indicates that selected bacterial strain have potential for controlling of soil-borne disease agents.