Abstract: Studies of some physical, chemical and biological parameters of the soil of taluka Rattodero, District Larkana were undertaken to investigate the occurrence of mycoflora from 45 soil samples of different depths of various locations. The mycoflora/dermatophytes are the sources of skin infections in animals and humans. Data presentation revealed different values of the physical and chemical analysis of soil pH, moisture, organic matter, nitrogen content and Total Dissolved Salts (TDS). During biological studies, various mycoflora including dermetophytes were investigated which were reported to be the cause of infection in laboratory animal and humans, respectively.