Abstract: The major aim of this investigation was to examine the effect of aqueous extract of Prasium majus`s L. leaves (AEPML) on intestinal influx of water and electrolytes by Everted Gut Sac (EGS). Four segments of rat intestine were detected and used (duodenum, jejunum, ileum and colon). The extract of the plant was used with two concentrations,8 and 16 g L-1, respectively. The results obtained showed that AEPML provoke; firstly an inhibition of water secretion by different parts of EGS, but decrease was only significant at the ileum (p<0.05).Secondly electrolytes fluxes were also sensitive to the addition of AEPML.On the other hand for Na+ influx,we noted an inhibition of the secretion at the duodenum and the colon (p < 0.01) with the dose 8 g L-1, while for Cl-, influx this inhibition was only registered at the colon (p<0.01) with the dose 8 g L-1. For K+ influx, AEPML induced rather a significant reabsorption at the duodenum, the jejunum and the colon (p < 0.01) hile at the ileum the reabsorption increased. This was only significant with the dose 16 g L-1.It may be concluding that the heterogeneity of the gastrointestinal tract in response to numerous factors which are able to affect homeostasis of water and electrolytes. AEPML might have an antisecretory function on water Na+ and Cl- and an absorptive function on K+.