Abstract: Production of the four targeted triterpenes, asiatic acid, madecassic acid, asiaticoside and madecassoside in leaf derived callus and cell suspension cultures of Centella asiatica was compared with its production in whole plant from field and in vitro shoot cultures. In callus a cultures, production was found the highest during the third week of culture and the contents declined gradually. The glycosides, madecassoside and asiaticoside contents were found higher than the asiatic acid and madecassic acid in callus cultures. Four triterpenes precursors, squalene, Farnesyl Pyrophosphate (FPP), Isopentenyl Pyrophosphate (IPP) and leucine, which are involved directly or indirectly in the triterpene biosynthetic pathway, were used to increase the triterpenes production. Squalene was found the best precursor in promoting triterpenes production in callus cultures. Squalene treated callus also produced the highest biomass production compared to the other precursors tested.