Abstract: The structural and biochemical study of apple bark splitting (ABS) disorder was conducted in Balochistan. Anatomical results revealed that the cells of the phloem were crushed and large lysigenous air cavities were formed. The cells of the vascular cambium were also crushed there was no contact between the cells of the vascular cambium and functional phloem or young xylem cells. The air cavities were present throughout the bark extended until cambial layers. The exude which was oozed out of the bark was phloem sap and rich in sugar and also contained little amount of proteins. The chemical analysis of the exude had high contents of Na, K, Mg, Fe, Ni and Co in ABS infected trees as compared to the healthy trees. However, the Cu content was same in both trees and Zn content was higher in exudates of the healthy tree as compared to ABS infected trees.