Abstract: Three wheat genotypes i.e. Bakhtawar-92, Punjab-96 and Inqilab-91 were tested for their response to callus induction frequency and their subsequent regeneration on a variety of media combinations. Bakhtawar-92 appeared to be the most responsive genotype to callus induction followed by Inqilab-91 and Punjab-96. It also produced significantly higher amount of callus as compared with other genotypes. However, the medium containing 2 mg l-1 2,4-D (2,4-dicholorophenoxy acetic acid) was found to be optimum for callus induction irrespective of the genotypes studied. Regeneration frequency of Bakhtawar-92 was 40% on the medium containing 0.1 mg l-1 IAA (Indole acetic acid) and 2.5 mg l-1 BAP (6-benzyl amino purine). Punjab-96 and Inqilab-91 showed regeneration of 25 and 33% on the medium supplemented with combination of 0.1 mg l-1 IAA and 0.5 mg l-1 BAP. Regenerated plants were evaluated for plant height, maturity and seed set. They had favourable significant differences from the control plants for the most important agronomic traits; plant height, days to maturity and kernels/spike etc.