Ionic Effect on Mobilization of Seed Storage Nutrient Substances and Lipase Activity in Germinating Oil Seeds (Brassica napus L.)
Abstract: Seeds storage nutrient substances like protein, fat and carbohydrate used up during germination for seedling growth. Brassica seeds (Brassica napus L) were germinated at various concentrations of NaCl and Na-Phosphate buffer up to 200 mM. Germinating levels, seedling length, degradation of seed storage substances and lipase activity were investigated. High salt concentrations resulted in retardation of seed germination compared with buffer and distilled water treatment. Degradation of storage substances like free sugar, reducing sugar, starch, lipid and protein were severely reduced by elevated concentration of salts doses higher than 100 mM and Na-Phosphate buffer doses higher than 150 mM, respectively. The mobilization of storage lipid in water and buffer treated Brassica seedling started about 22 and 32 h respectably after imbibitions but salts treated germinating Brassica seedling delayed about 48 h after imbibitions. Lipase activity inhibited by salts treatment was confirmed by using triolein as a substrate.
Ionic Effect on Mobilization of Seed Storage Nutrient Substances and Lipase Activity in Germinating Oil Seeds (Brassica napus L.). Pakistan Journal of Biological Sciences, 7: 36-41.