Abstract: The aim of the present study was to investigate the biocidal activity of seven new chromium based coordination complexes [Cr(Pht)2 (Glycine)2, S1], [Cr(Pht)2 (Leucine)2, S2], [Cr(Pht)2 (Cystein)2, S3], [Cr(Pht)2 (Serine)2, S4], [Cr(Suc)2 (Leucine)2, S5], [Cr(Suc)2 (Cystein)2, S6] and [Cr(Suc)2 (Serine)2, S7] against Gram-positive and -negative bacteria, fungi and brine shrimp nauplii. The complexes S1, S3, S4, S6 showed good antibacterial activity at the concentration of 200 μg disc-1 and gave MIC values between 16-64 μg ml-1 against the tested bacteria. The complexes gave comparatively better antibacterial activity against the Gram-negatives. S2 did not show any remarkable antifungal activity but others showed good activity. The brine shrimp were hatched in artificial sea water and exposed to the complexes to determine mortality rate. LC50 values were calculated after probit transformation of the resulting data. All the complexes showed better cytotoxic effect but among them S1 and S3 having LC50 values of 3.31 and 3.63 μg ml-1 showed potent cytotoxic activity when compared with the reference standard Gallic acid whose LC50 values was 4.53 μg ml-1.