Abstract: Ionic effect on the degradation of oil seeds (Brassica napus L) storage lipid was investigated during germination. Lipid degradating enzyme lipase was identified in germinating Brassica napus L and the enzyme was purified to homogeneity by extraction, Sephadex G-50, DEAE and CM-cellulose column chromatography. The enzyme was purified to 67.59 fold with the final specific activity of 39.10 mmol min-1 mg-1 at 37°C using triolein as a substrate. The purified enzyme showed a single band when it was subjected to SDS / PAGE electrophoresis. The SDS/PAGE electrophoresis indicated a molecular mass of 34 kDa for this lipase which corresponding to the high-resolution gel filtration. The optimum pH and temperature for hydrolysis of Oleic acid were 7.0 and 37°C, respectively. In the presence of Ca 2+ and Bi 3+, the lipase activity was dramatically enhanced by 165 and 124%, respectively. Fe3+, Fe2+, Zn2+, Hg2+ and Cu2+ could inhibit this lipase but Al 3+, Pb 2+ showed no influence on hydrolysis activity. Km of this enzyme was 0.23 mM.