Abstract: Using synthetic enrichment medium, fifteen bacterial colonies were isolated, purified, preserved and stained for Poly-β-Hydroxybutyric Acid (PHB) granules with Sudan Black B stain. Among them ten showed PHB positive reaction. An attempt was made for the production, isolation and purification of polymer by the proposed isolate QGR. 7.81% of biodegradable polymer production was recorded by QGR. On the basis of cultural, morphological, physiological and biochemical characteristics the isolate QGR was provisionally identified as Listeria murrayi. The chloroform extract of biodegradable polymer of isolate QGR was analyzed by Gas Chromatography Linked to Mass Spectroscopy (GCMS) where it showed eighteen different compounds. Among them, ethyl cyclopropane carboxylate, oleic acid and di-n-octylphthalate are the major compounds.