Abstract: Carbon dioxide (CO2) enriched atmospheres were significantly effective to inhibit fungi growth in vitro and to extend the shelf life of banana fruits during storage at 20°C. Thirteen postharvest fungi were treated daily with each of the following: (a), 2, 5, 10, 20, 40, 60 kPa CO2; (b) continuously with 10, 20, 40, 60 kPa CO2 at 20 kPa oxygen (O2) and (c) exposed only to 1 and 0.1 kPa O2. The fungi were classified into high, medium and low sensitivity groups based on their growth response to CO2, applied either by daily or continuously flushing. Low concentrations of CO2 (2-20 kPa) showed no significant inhibition of fungal growth, while high CO2 (40-60%) significantly suppressed the mycelial growth of almost all fungi of the high, medium and low sensitivity groups.