Abstract: Five phytopathogenic strains namely, Xanthomonas oryzae NA1, Xanthomonas citri NA2, Pseudomonas andropogonis NA3, Erwinia carotovora NA4 (isolated from diseased fruits and vegetables) and Agrobacterium radiobacter NA5 (isolated from pepper rhizophere) were identified on morpho-cultural and biochemical considerations. API 20 E and API 20 NE kits were used to confirm the identification. All isolates were screened for bacteriocinogenic activity against phytopathogenic bacteria. Accordingly, only two strains i.e. Erwinia carotovora NA4, Agrobacterium radiobacter NA5 were found to produce bacteriocin. These bacteriocins are designated as erwiniocin NA4, agrocin NA5, respectively. Both the producer strains have shown antibacterial activity against closely related strains. The activity potential of erwiniocin NA4 was calculated as 160AU mL-1 while that of agrocin NA5 was 80AU mL-1. The effect of temperature variation and pH on erwiniocin NA4 and agrocin NA5 was also checked and both were found activity resistant at 100°C for 10 min and pH range 2-14. Erwiniocin NA4 was also found resistant to autoclaving while bioactivity of agrocin NA5 was lost after similar treatment.