Abstract: The present study was conducted with an aim of establishing the best organic solvent system that can yielded the Channa striatus extract with highest antinociceptive activity and also to determine the polarity of the respective bioactive compound using the acetic acid- induced abdominal constriction test. Briefly, the Channa striatus fillet were extracted separately with methanol, ethanol, chloroform, chloroform:methanol and distilled water overnight. This procedure is repeated three times and each of the supernatant obtained was pooled together and then subjected to the evaporation process. The extracts obtained was prepared in the doses of 0.5, 1.0, 2.0, 4.0 and 8.0 g kg-1 and administered subcutaneously (SC) into mice. Thirty min after the administration of extracts, 0.6% acetic acid was administered intraperitoneally (IP). All solutions were administered in the volume of 10 mL kg-1. From the data obtained, it can be concluded that the bioactive compounds to be of two types, the polar compound that is water-soluble and the non-polar compound that is fat-soluble. These two compounds might act alone, as in DH2O and chloroform extracts or act together, as in CM, methanol and ethanol extracts. Furthermore, the ability of this compound(s) to exhibit the antinociceptive activity in all the three types of solvents also suggested the presence of peptide compound. However, further investigation need to be carried out in order to isolate and identify the respective compound. In addition, methanol and chloroform:methanol were found to be the best solvents for further isolation of the respective bioactive compound of Channa striatus.