Abstract: In this study, ethyl acetate, acetone, chloroform and ethanol extracts of Cantharellus cibarius Fr. (Cantharellaceae) were tested for antimicrobial activity by Disc Diffusion method on the following test microorganisms : Aeromonas hydrophila ATCC 7966, Listeria monocytogenes ATCC 19117, Escherichia coli ATCC 11230, Enterobacter aerogenes ATCC 13048, Corynebacterium xerosis CCM 2824, Corynebacterium glutamicum ATCC 13022, Proteus vulgaris ATCC 8427, Serratia marcescens NRRL 3284, Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, Bacillus brevis ATCC 9999, Bacillus sphaericus, Bacillus megaterium, Mycobacterium smegmatis CCM 2067, Sarcina lutea, Micrococcus luteus LA 2971, Staphylococcus aureus ATCC 6538P, Stapylococcus epidermidis NRRL B-4877, Alcaligenes faecalis CCM 3763, Alcaligenes eutrophus, Salmonella parathyphi B, Salmonella typhi ATCC 19430, Salmonella tyhimurium CCM 5445, Klebsiella pneumoniae UC57, Micrococcus roseus, Micrococcus flavus ATCC 14452, Citrobacter freundii ATCC 8090, Bordatella bronchiseptica ATCC 4617, Erwinia amylovora, Xanthomonas campestris, Pseudomonas extorquens, Pseudomonas fluorescens, Pseudomonas aeruginosa ATCC 27853, Pseudomonas putida, Kluyveromyces fragilis NRRL 2415, Candida albicans ATCC 10231, Candida utilis LA 991, Hansenula sp., Rhodotorula rubra, Debaryomyces sp., Saccharomyces cerevisiae ATCC 9763, Schizosaccharomyces sp., Torulopsis sp., Torula sp., Aspergillus oryzae, Aspergillus flavus, Botrytis cineriae, Fusarium oxysporium, Streptomyces murinus ISP 5091 and Nocardia cornea IFO 14403. From the present study it was found that Cantharellus cibarius Fr. revealed antimicrobial activity against some Gram (+) and Gram (-) bacteria, yeasts, filamentous fungi and actinomycetes also used in this study.