Abstract: Barley leaf stripe caused by Pyrenophora graminea was detected for the first time in dryland district of Turkey. With the approximate 3.2% infection rate, the first symptom of the pathogen was detected at the tillering growth stage in 2002 growing season. In second surveys conducted at the milk-ripe stage of Angora barley cv, disease severity was measured approximately 5.3% as a sum. In this assessment, diseased plants were displayed three differ infection type as physiologically in the following; plants without ears, plants with ears but without seeds and plants with ears having deformed seeds. Rates of these symptoms were approximately 2.8, 1.5 and 1%, respectively. The yield loss from leaf stripe was estimated as 5% in 2002. When same crops were treated with experiments in 2003, 9.6% of seeds were displayed P. graminea in vitro tests, while 7.9% of plants demonstrated this infection in field experiments. According to the present results, it is suggested that the direct effect of leaf stripe on yield lose was minor but, it was very important when quarantine conditions are taken into consideration.