Abstract: The research was conducted with three triticale cultivars planted in randomized block design in three replications in 2002-2003 growing season under Central Anatolian conditions of Turkey. It was studied to estimate the grain number per spike, percentage of seed set and thousand-grain weight in spontaneous cross-pollinated, self-pollinated and untreated spike. It was determined that the seed set of spontaneous cross-pollinated, self pollinated and untreated spike varied from 57.6-64.33%, 65.36-81.80% and 84.23-88.10%, respectively. The highest thousand-grain weight amongst triticale genotypes used in the trial was found in untreated spikes (mean; 40.15 g).