Abstract: Three coordination complexes of metals with mixed ligands [Mn(ED)(2-ap)2, A], [Fe(ED)(2-ab)2, B] and [Fe(ED)(2-ap)2, C] were tested for their antimicrobial activity by disc diffusion and serial dilution methods. All the complexes were active against various test pathogenic organisms. The maximum antibacterial and antifungal activities were shown by the manganese complex A. The minimum inhibitory concentration of the complex A was determined against four pathogenic bacteria Bacillus subtilis, Streptococcus-β-haemolyticus,Escherichia coli and Salmonella typhi and values were found between 16-32 μg ml-1. The manganese complex A also showed potent cytotoxic effect which is indicative of its probable effect on cancer cell lines.