Abstract: Studies on the solublisation of copper from chalcopyrite by direct (contact)and indirect leaching using Thiobacillus ferrooxidan were carried out. Thiobacillus ferrooxidan produced up to 2380 ppm acidic Fe+3 solution of pH 1.5 in 480 h. The direct leaching method was slow for the solublisation of copper from the ore and only 28.52% of copper leached in 70 days. While on the other hand 87.57% of copper was brought in to solution in 192 h by indirect leaching technique utilizing biologically oxidized acidic Fe+3 solution at 30 C. The increase in temperature of the indirect leaching process, shorten the metal recovery time. At 60°C maximum leaching (88.30%) was obtained in 8 hrs only.