Abstract: In this study, fertilization biology of Burdur dimriti, Sariemin, Tilki kuyrugu, Razaki, Buzgulu, Siyah buzgulu and Siyah gemre grape varieties (Vitis vinifera L.) which are grown in Isparta, Turkey ecological conditions was investigated. For this purpose, rates of fruit set obtained from open pollination and isolation experiment were determined in vineyard. Furthermore, pomological analyses were done to determine the effects of experiments on fruit quality. It was determined that fruit set levels varied between 30.0 and 40.6% in open pollination, 15.0 and 34.3% in isolation experiment. In the study, Siyah dimrit, Sariemin, Tilki kuyrugu, Razaki and Siyah gemre varieties with hermaphrodite flowers were self-fertile, but Siyah buzgulu variety having hermaphrodite flower was found partially self-fertile and Burdur dimriti with hermaphrodite flowers and Buzgulu with morphological hermaphrodite-physiological female flowers were found self-unfertile.