Abstract: In order to determine the potentials of in vitro gynogenetic induction via in vitro ovary culture in tomato a total of 358 ovaries were cultured. Flower buds, of six different cultivars, containing microspores at the uninucleate stage were removed from the plants and following an initial pretreatment on a solid 0.3 M mannitol starvation medium ovaries were cultured on four different media containing 1/2 MS as the basic medium together with either kinetin riboside or BAP as cytokinins, both in two concentrations, being in combination with the IAA as the auxin also in two concentrations. Ovaries were subcultured on two media, i.e. NLN+2,4-D 1.11 mg l-1+Glycine 500 mgl/l and NLN+NAA 0.93 mg l-1 + Casein Hydrolysae 5 g l-1, to induce further sporophytic development. Regeneration neither of somatic nor of gametophytic origin were observed. During culture, ovary walls turned brown and withered but the ovules carried were of two types: one was of globular shape and when observed microscopically showed living cell clumps within the embryo sacs and the other was of almost rectangular shape comprising shrunk ovules containing dead embryo sac cells within the ovules.