Abstract: Haploid induction potentials in Lycopersicon esculentum Mill. (2n=2x=24) via unilateral pollination with Solanum sisymbriifolium Lam. (2n=2x=24) pollen were investigated. In April, the cv. Invictus set 12 fruits out of 72 pollinations and, the remaining six cultivars did not set fruit. Whereas in May, from a total of 242 pollinations onto eight varieties, 26 fruits were obtained from only six of the varieties. In addition to the ovules obtained from the cv. Invictus fruits, cv. Sagit 146 fruits contained 65 ovules. While cv. Invictus ovules gave rise to plantlets, ovules from cv. Sagit 146 were lost due to contamination. Chromosome counting of eight in vitro plants of cv. Invictus showed cells with 24, 25 and 26 chromosomes. Haploid chromosome numbers were not observed in any of the cells studied. Potentials of the approach were also discussed.