Abstract: The study was aimed at assessing the frequency of Microsporum gypseum, a potentially pathogenic dermatophyte in various samples collected from poultry farm of Rewa, Madhya. Pradesh, India. The fungal flora was analysed in different samples for the presence of Microsporum gypseum by Hair Baiting Technique as recommended by Vanbreuseghem. The isolated strains of M. gypseum were cultured on Sabouraud`s Dextrose Agar supplemented with cycloheximide and chloramphenicol for at least 3 weeks and then identified on the basis of their microscopic and macroscopic morphology. A total of 37 samples were observed for the presence of this fungi and out of them, 25 were found positive for the occurrence of M. gypseum (67.56%).The highest frequency of M. gypseum was observed in the 3rd type of samples which were taken between the months of August and February when the temperature and relative humidity is almost favourable. On the other hand, the rest of the samples i.e., 1st 2nd and 4th type were taken and observed during April, May and March, respectively showing less frequency of M. gypseum. This shows clearly that the frequency of Microsporum gypseum is related with the infection caused by this fungi in Rewa (M.P.), India.