Abstract: The present study was to evaluate the effect of breed and intervals between collection on semen characteristics. Semen was collected from eight breeding bulls belonging to four genetic groups (100%SL, 75%F×25%L, 50%SL×50%F and 50%F×50%L). Genetic groups had a significant effect (P<0.05) on semen characteristics except motility and total number of semen collection per month. The highest sperm concentration, total number of motile sperm cells/jaculate and total number of semen doses per collection were found in 100%SL and lowest in 50%F×50%L. Similarly, intervals between collection significantly (P<0.05) affected all the semen traits except volume of ejaculate and total number of semen doses per ejaculate. The highest sperm concentration (1332±29 mill. Ml-1), total number of motile sperm cells/jaculate (5621±261.99 million), motility (64±0.24%) and total number of collection per month (5.84±0.23) was obtained with an interval <6 days between collection than the other interval groups. It was concluded that 100% Sahiwal and 50% Sahiwal ×50% Friesian for the genetic group and <6 days for the interval between collection were suitable for most of the semen parameters than all other groups.