Abstract: Two chromium resistant bacterial strains i.e. Bacillus sp. S6 and Staphylococcus sp. CrM-3 were used in conjunction with Eichornia crassipes to study the effect of chromate removal from chromium contaminated waste waters. Bacterial strains resulted in reduced uptake of chromate into inoculated plants, which was 17.24 and 11.29% with S6 and CrM-3 respectively, at an initial Cr (VI) concentration of 100 μg ml‾1 as compared to non-inoculated control plants. Different heavy metals (CuSO4, NiSO4, MnCl2 and Pb (NO3) 2) at a concentration of 100 μg ml‾1 were added in chromate-supplemented solution to check their effects on chromate uptake by the plants. At different chromate concentrations the uptake of Cr (VI) into the plants was 28.73, 5.38, 7.15, 12.29 and 15.35% less at an initial Cr (VI) concentration of 100, 300, 500, 1000 and 2000 μg ml‾1 in comparison to metal free condition. Cr (VI) uptake by the Eichornia crassipes was occurred at different pH tested, but maximum uptake was observed at pH 5. Nevertheless the bacterial strains caused some decrease in chromate uptake into the plants but the combined effect of plants and bacterial strains conduce more removal of Cr (VI) from the solution.