Abstract: Mercury concentration in seafood from Pakistan coast is being reported. For this purpose ten different fish specie were examined for the analysis of Mercury. Mercury concentration in prawn and crab specie was also determined. The samples were collected from coastal area of Karachi, namely Vindar, from where it is not only supplied to local markets but also exported to different countries. Mercury concentration in muscles of Mushka (Pseudosciena sina) is found to be higher as compared to other specie. Determination of Mercury has been carried out by Cold Vapor Atomic Absorption Spectroscopy.