Abstract: Two wild strains of Barbodes gonionotus obtained from Indonesia and Thailand and an existing stock were used to develop a crossbred (F3) through selective breeding using 3 x 3 diallele crossing method and characterized by morphometric, meristic and growth study. Morphometric studies showed that there was no significant difference among the strains. Relationship of body length with body weight, head height, head length, body width, caudal peduncle length, pectoral fin length and pelvic fin length of these four strains was linear. The crossbred gave significantly (P<0.05) higher value for anal fin rays than that recorded for other three strains. Three days old larval size was significantly different among the strains, Bangla and crossbred showed higher length than that of others. In frequency distribution analysis of 3 days old larval size showed comparatively great percentage of lower length group (3 mm) in Thai and Indo strain and comparatively great percentage of higher length group (4.5 mm) in Bangla and crossbred strain. Bangla and crossbred showed better quality performance among the four strains. In outdoor growth (g/day), crossbred (0.0057) showed higher than that of Bangla strain (0.0043) but they were not significantly different. Survival rate of crossbred (12%) also higher than that of Bangla (10%) strain. Several desired effects of different characters of crossbred in general observed in the present experiment have tempted to conclude that this stock may economically be cultured in Bangladesh.